Molecular Mechanism of the Qiang-Xin 1 Formula on Sepsis-Induced Cardiac Dysfunction Based on Systems

phase1tox

Uncovering the Molecular Mechanism of the Qiang-Xin 1 Formulation on Sepsis-Induced Cardiac Dysfunction Primarily based on Techniques Pharmacology

 

Cardiac dysfunction is a crucial manifestation of sepsis-induced multiorgan failure and ends in the excessive mortality of sepsis. Our earlier examine demonstrated {that a} conventional Chinese language medication formulation, Qiang-Xin 1 (QX1), ameliorates cardiac tissue harm in septic mice; nonetheless, the underlying pharmacology mechanism stays to be elucidated. The current examine was geared toward clarifying the protecting mechanism of the QX1 formulation on sepsis-induced cardiac dysfunction. The reasonable sepsis mannequin of mice was established by cecal ligation and puncture surgical procedure.
Therapy with the QX1 formulation improved the 7-day survival consequence, attenuated cardiac dysfunction, and ameliorated the disruption of myocardial construction in septic mice. Subsequent programs pharmacology evaluation discovered that 63 bioactive compounds and the associated 79 candidate goal proteins have been screened from the QX1 formulation.
The community evaluation confirmed that the QX1 energetic elements quercetin, formononetin, kaempferol, taxifolin, cryptotanshinone, and tanshinone IIA had a superb binding exercise with screened targets. The integrating pathway evaluation indicated the calcium, PI3K/AKT, MAPK, and Toll-like receptor signaling pathways could also be concerned within the protecting impact of the QX1 formulation on sepsis-induced cardiac dysfunction.
Additional, experimental validation confirmed that the QX1 formulation inhibited the exercise of calcium/calmodulin-dependent protein kinase II (CaMKII), MAPK (P38, ERK1/2, and JNK), and TLR4/NF-κB signaling pathways however promoted the activation of the PI3K/AKT pathway. A cytokine array discovered that the QX1 formulation attenuated sepsis-induced upregulated ranges of serum IFN-γ, IL-1β, IL-3, IL-6, IL-17, IL-4, IL-10, and TNF-α.
Our information prompt that QX1 could signify a novel therapeutic technique for sepsis by suppressing the exercise of calcium, MAPK, and TLR4/NF-κB pathways, however selling the activation of AKT, thus controlling cytokine storm and regulating immune stability.
The current examine demonstrated the multicomponent, multitarget, and multipathway traits of the QX1 formulation and offered a novel understanding of the QX1 formulation within the scientific software on cardiac dysfunction-related ailments.
phase1tox
phase1tox

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Human Apolipoprotein C-III protein control for WB

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Purified human Catalase (CATL) protein control for WB

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copy number positive control for custom human assay

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Mouse antibody for Hepatitis C virus NS4a+b

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genesig Standard kit for Hepatitis B Virus v2.0

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genesig Standard kit for Hepatitis C Virus v2.0

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Formamide, GlenBiol™, suitable for molecular biology

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Control Peptide for SNAPtide® #521

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Human plasma Apolipoprotein E protein control for WB

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EUR 343.2

Human Plasma Apolipoprotein J protein control for WB

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Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)

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Mouse antibody for Hepatitis B virus sAg. probe

1837 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus sAg probe for WB, ELISA.

Mouse antibody for Hepatitis B virus sAg. probe

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EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus sAg probe for WB, ELISA.

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Purified, recombinant Human TACE control protein for WB

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Extraction Control for Challenging Samples

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Description: This is purified Mouse monoclonal antibody against Hepatitis B virus X protein for WB, ELISA.

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Description: This is purified Mouse monoclonal antibody against Hepatitis B virus X protein for WB, ELISA.

Mouse antibody for Hepatitis B virus X protein

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EUR 386.26
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Mouse antibody for Hepatitis B virus e antigen

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EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus e antigen for WB, ELISA.

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Description: This is purified Mouse monoclonal antibody against Hepatitis B virus e antigen for WB, ELISA.

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Description: This is purified Mouse monoclonal antibody against Hepatitis B virus e antigen for WB, ELISA.

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ELISA kit for Mouse Hepatitis Virus IgG (HV IgG)

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EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Mouse Hepatitis Virus IgG (HV IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Hepatitis Virus IgG (HV IgG)

KTE71599-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Mouse Hepatitis Virus IgG (HV IgG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Prothrombin (Factor II) protein control for Western

PRTN11-C 100 ul
EUR 343.2

Mouse antibody for Hepatitis B virus sAg. capture

1834 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus sAg capture for WB, ELISA.

Mouse antibody for Hepatitis B virus sAg. capture

1835 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus sAg capture for WB, ELISA.

Mouse antibody for Hepatitis B virus sAg. capture

1836 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus sAg capture for WB, ELISA.

Flow Control Fitting for Blowgun - EACH

FLY1252 EACH
EUR 91.07

Negative Control for Ms IgG1, Antibody

GWB-B79944 7 ml Ask for price

Negative Control for Ms IgG1, Antibody

GWB-D18CD7 1 ml Ask for price

Human Angiopoietin 4 (Ang-4) protein control for WB

ANG41-C 100 ul
EUR 343.2

NATtrol Respiratory Panel 2.1 (RP2.1) Controls (12 x 0.3mL)

NATRPC2.1-BIO 12 x 0.3mL
EUR 529

Mouse antibody for Hepatitis B virus core antigen

1822 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus core antigen for WB, ELISA.

Mouse antibody for Hepatitis B virus core antigen

1823 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus core antigen for WB, ELISA.

Mouse antibody for Hepatitis B virus core antigen

1824 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Hepatitis B virus core antigen for WB, ELISA.

Negative Control for Ms IgG2a, Antibody

GWB-2AA549 7 ml Ask for price

Negative Control for Ms IgG2a, Antibody

GWB-DB66F7 1 ml Ask for price

Inhibition Control for EZ‐Series Assay

TC-9110-001 each
EUR 75

Insulator Primarily based Dielectrophoresis: Micro, Nano, and Molecular Scale Organic Functions

 

Insulator primarily based dielectrophoresis (iDEP) is turning into more and more essential in rising biomolecular functions, together with particle purification, fractionation, and separation.
In comparison with standard electrode-based dielectrophoresis (eDEP) strategies, iDEP has been demonstrated to have the next diploma of selectivity of organic samples whereas additionally being much less biologically intrusive.
Over the previous 20 years, substantial technological advances have been made, enabling iDEP to be utilized from micro, to nano and molecular scales. Comfortable particles, together with cell organelles, viruses, proteins, and nucleic acids, have been manipulated utilizing iDEP, enabling the exploration of subnanometer organic interactions.
Current investigations utilizing this system have demonstrated a variety of functions, together with biomarker screening, protein folding evaluation, and molecular sensing. Right here, we overview present state-of-art analysis on iDEP programs and spotlight potential future work.

Molecular Survey of Respiratory and Immunosuppressive Pathogens Related to Low Pathogenic Avian Influenza H9N2 Subtype and Virulent Newcastle Illness Viruses in Industrial Hen Flocks

 

The examine was carried out in 48 poultry flocks to elucidate the roles of assorted complicating pathogens concerned together with Newcastle illness (ND)/ low pathogenic avian influenza (LPAI) outbreaks.
Necropsy was performed and samples have been collected for the isolation of Newcastle illness virus (NDV), Influenza A virus, infectious bronchitis virus (IBV), pathogenic micro organism; molecular detection of infectious laryngotracheitis virus (ILTV), fowl adeno virus (FAV), rooster anaemia virus (CAV), Mycoplasma synoviae (MS) and Mycoplasma gallisepticum (MG).
The isolation outcomes confirmed that 18/48 flocks (37%) have been constructive for the presence of hemagglutinating brokers. Out of 18 hemagglutination (HA) constructive flocks, 11 flocks (61%) have been constructive for each avian influenza virus (AIV) and NDV; Four flocks (22%) have been constructive for NDV; and three flocks (17%) have been constructive for AIV. Sequence evaluation of hemagglutinin and neuraminidase genes of AIV revealed that every one have been belonging to LPAI-H9N2 subtype.
Sequence evaluation of F gene of NDV revealed that they belong to virulent kind. The PCR outcomes confirmed the presence of three to seven etiological brokers (CAV, FAV, ILTV, MG, MS and avian pathogenic E. coli together with LPAI/NDV from all of the 18 HA-positive flocks.
The detection charge of triple, quadruple, quintuple, sextuple and sevenfold infections was 17% (Three flocks), 28% (5 flocks), 11%, (2 flocks) 28% (5 flocks) and 17% (Three flocks), respectively.
In conclusion, the illness complicated concerned multiple pathogen, primarily ensuing from the interaction between LPAI-H9N2 and NDV; subsequently this may very well be exacerbated by co-infection with different brokers which can trigger exacerbated outbreaks which will in any other case go undetected in area.

Herpes Simples Virus 2 IgM Control Serum

C1052M 3 mL
EUR 105

Mouse antibody for Herpes simplex virus

194 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA.

Mouse antibody for Herpes simplex virus

195 100 ug
EUR 425.4
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA.

Mouse antibody for Herpes simplex virus

197 100 ug
EUR 425.4
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA.

Mouse antibody for Herpes simplex virus

199 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA.

Mouse antibody for Herpes simplex virus

190 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA.

Mouse antibody for Herpes simplex virus

128 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 gG for WB, ELISA.

Mouse antibody for Herpes simplex virus

129 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 gG for WB, ELISA.

Mouse antibody for Herpes simplex virus

116 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 gG for WB, ELISA.

Mouse antibody for Herpes simplex virus

115 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 nuclear for WB, ELISA.

Mouse antibody for Herpes simplex virus

119 100 ug
EUR 425.4
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 nuclear for WB, ELISA.

Mouse antibody for Herpes simplex virus

117 100 ug
EUR 425.4
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 Thymidine kinase for WB, ELISA.

Mouse antibody for Herpes simplex virus

112 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 1 gE for WB, ELISA.

Goat polyclonal antibody for Herpes Simplex virus

131 1 ml
EUR 327.53
Description: This is goat polyclonal antibody against Herpes Simplex virus type 1 for WB, ELISA.

Goat polyclonal antibody for Herpes Simplex virus

124 1 ml
EUR 373.2
Description: This is HRP conjugated goat polyclonal antibody against Herpes Simplex virus type 1 for WB, ELISA.

Mouse antibody for Herpes simplex virus 1&2

196 100 ug
EUR 431.93
Description: This is Mouse monoclonal FITC conjugated antibody against Herpes simplex virus, 1&2 gD for WB, ELISA.

Sheep polyclonal antibody for Herpes Simplex virus

127 1 ml
EUR 347.1
Description: This is Biotin conjugated sheep polyclonal antibody against Herpes Simplex virus type 2 for WB, ELISA.

Sheep polyclonal antibody for Herpes Simplex virus

121 1 ml
EUR 327.53
Description: This is sheep polyclonal antibody against Herpes Simplex virus type 2 for WB, ELISA.

Sheep polyclonal antibody for Herpes Simplex virus

123 1 ml
EUR 347.1
Description: This is FITC conjugated sheep polyclonal antibody against Herpes Simplex virus type 2 for WB, ELISA.

Mouse antibody for Herpes simplex virus type 2

113 100 ug
EUR 431.93
Description: This is Mouse monoclonal FITC conjugated antibody against Herpes simplex virus, type 2 gE for WB, ELISA.

genesig Easy kit for Herpes simplex type 1 (HHV1)

Z-Path-HSV1-EASY 50 tests
EUR 410
Description: HSV1

genesig Easy kit for Herpes simplex type 2 (HHV2)

Z-Path-HSV2-EASY 50 tests
EUR 410
Description: HSV2

ELISA Kit for Antibody IgM to Herpes Simplex Virus Type II

Z7010006 1 kit
EUR 284

NATIVE HERPES SIMPLEX VIRUS 2

GWB-614440 1 ml Ask for price

NATIVE HERPES SIMPLEX VIRUS 1

GWB-8E3AF9 1 ml Ask for price

Herpes simplex type 1

Oneq-H426-OneqH426100D Oneq-H426-100D
EUR 515

Herpes simplex type 1

Oneq-H426-OneqH426150D Oneq-H426-150D
EUR 594

Herpes simplex type 1

Oneq-H426-OneqH42650D Oneq-H426-50D
EUR 413

Herpes simplex type 2

Oneq-H427-OneqH427100D Oneq-H427-100D
EUR 515

Herpes simplex type 2

Oneq-H427-OneqH427150D Oneq-H427-150D
EUR 594

Herpes simplex type 2

Oneq-H427-OneqH42750D Oneq-H427-50D
EUR 413

Herpes simplex type 1

PCR-H426-PCRH42648D PCR-H426-48D
EUR 230

Herpes simplex type 1

PCR-H426-PCRH42696D PCR-H426-96D
EUR 312

Herpes simplex type 2

PCR-H427-PCRH42748D PCR-H427-48D
EUR 230

Herpes simplex type 2

PCR-H427-PCRH42796D PCR-H427-96D
EUR 312

Herpes Simplex Virus Antibody

GWB-244DEA 1 ml Ask for price

Herpes Simplex Virus-1 gD

PR27127 500 ug
EUR 999.6

Herpes Simplex Virus-1 gG

PR27128 500 ug
EUR 999.6

Herpes Simplex Virus-2 gD

PR27129 500 ug
EUR 999.6

Herpes Simplex Virus-1 gD

PR27127-B each
EUR 540

Herpes Simplex Virus-1 gG

PR27128-B each
EUR 540

Herpes Simplex Virus-2 gD

PR27129-B each
EUR 540

Herpes Simplex Virus-2 gG

rAP-5322 Inquiry Ask for price

Herpes simplex virus, type 1

9010 each
EUR 440
Description: glycoprotein gD

Herpes simplex virus, type 2

9011 each
EUR 440
Description: gycoprotein gD

Herpes simplex virus, type 1

9014 each
EUR 370
Description: glycoprotein G

HERPES SIMPLEX VIRUS 1 Protein

GWB-Q00415 1 ml Ask for price

HERPES SIMPLEX VIRUS 2 Protein

GWB-Q00417 1 ml Ask for price

HERPES SIMPLEX VIRUS 2 Protein

GWB-Q00418 0.2 ml Ask for price

Herpes Simplex Virus 1 Antigen

HS105-1000 1.0
EUR 1178.79
Description: HSV-1 particles from a cell lysate are purified by sucrose gradient ultracentrifugation.

Herpes Simplex Virus 1 Antigen

HS105-200 0.2
EUR 294.7
Description: HSV-1 particles from a cell lysate are purified by sucrose gradient ultracentrifugation.

Herpes Simplex Virus 2 Antigen

HS205-1000 1.0
EUR 1178.79
Description: HSV-2 particles from a cell lysate are purified by sucrose gradient ultracentrifugation.

Herpes Simplex Virus 2 Antigen

HS205-200 0.2
EUR 294.7
Description: HSV-2 particles from a cell lysate are purified by sucrose gradient ultracentrifugation.

Herpes simplex Virus 1 Antigen

BA1051VS 1mg
EUR 877

Herpes simplex Virus 2 Antigen

BA1052VS 1mg
EUR 877

Herpes simplex Type-2 Antibody

GWB-5A8586 1 ml Ask for price

Herpes simplex Type-2 Antibody

GWB-43A7C8 1 ml Ask for price

Herpes simplex Type-2 Antibody

GWB-194C60 1 ml Ask for price

Herpes simplex Type-1 Antibody

GWB-B28FC0 1 ml Ask for price

Herpes simplex Type-1 Antibody

GWB-5EB3C0 1 ml Ask for price

Herpes Simplex Virus gG2 Protein

HSV-gG2-100 0.1
EUR 473.31
Description: Native HSV2 gG2 protein purified from HSV2 infected Baby Hamster Kidney (BHK) cells.

Herpes Simplex Virus gG2 Protein

HSV-gG2-1000 1.0
EUR 1422.47
Description: Native HSV2 gG2 protein purified from HSV2 infected Baby Hamster Kidney (BHK) cells.

Herpes simplex Type-1 Antibody

GWB-8EEBAC 1 ml Ask for price

Herpes Simplex Virus 1 (HSV 1)

GWB-7E1F5B 1 ml Ask for price

Herpes Simplex Virus 2 (HSV 2)

GWB-814A13 1 ml Ask for price

Herpes Simplex Virus 2 (HSV 2)

GWB-FA122B 1 ml Ask for price

Herpes Simplex Virus 1 (HSV 1)

GWB-D60086 1 ml Ask for price

Herpes simplex type 1 PCR kit

PCR-H426-48D 50T
EUR 543.6

Herpes simplex type 1 PCR kit

PCR-H426-96D 100T
EUR 686.4

Herpes simplex type 2 PCR kit

PCR-H427-48D 50T
EUR 543.6

Herpes simplex type 2 PCR kit

PCR-H427-96D 100T
EUR 686.4

Herpes Simplex 1 IgA ELISA kit

55R-IB79241 96 wells
EUR 382.8
Description: ELISA kit for the detection of Herpes Simplex 1 IgA in the research laboratory

Herpes Simplex 1 IgG ELISA kit

55R-IB79242 96 wells
EUR 379.2
Description: ELISA kit for the detection of Herpes Simplex 1 IgG in the research laboratory

Herpes Simplex 1 IgM ELISA kit

55R-IB79243 96 wells
EUR 379.2
Description: ELISA kit for the detection of Herpes Simplex 1 IgM in the research laboratory

Herpes Simplex 2 IgA ELISA kit

55R-IB79247 96 wells
EUR 379.2
Description: ELISA kit for the detection of Herpes Simplex 2 IgA in the research laboratory

Herpes Simplex 2 IgG ELISA kit

55R-IB79248 96 wells
EUR 379.2
Description: ELISA kit for the detection of Herpes Simplex 2 IgG in the research laboratory

Herpes Simplex 2 IgM ELISA kit

55R-IB79249 96 wells
EUR 379.2
Description: ELISA kit for the detection of Herpes Simplex 2 IgM in the research laboratory

Herpes Simplex Virus 2 Antibody (gG)

GWB-374257 1 mg Ask for price

Herpes Simplex Virus-2 gG Protein

20-abx260324
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Herpes Simplex Virus-1 gD Protein

20-abx260325
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Herpes Simplex Virus-2 gD Protein

20-abx260326
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Herpes Simplex Virus-2 gG Protein

20-abx260327
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Herpes Simplex Virus-1 gG Protein

20-abx260328
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Herpes Simplex Virus-2 gB Protein

20-abx260330
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Herpes Simplex Virus 1 Antibody (gC)

GWB-971A5F 0.2 mg Ask for price

Herpes Simplex Virus 2 Antibody (gD)

GWB-90CF2E 0.2 mg Ask for price

Herpes Simplex Virus 1 Antibody (gB)

GWB-927CCF 0.1 mg Ask for price

Herpes Simplex Virus 2 Antibody (gC)

GWB-F62608 0.2 mg Ask for price

Herpes Simplex Virus-2 gG Protein

abx260324-20g 20 µg
EUR 950

Herpes Simplex Virus-2 gG Protein

abx260324-5g 5 µg
EUR 350

Herpes Simplex Virus-1 gD Protein

abx260325-20g 20 µg
EUR 950

Herpes Simplex Virus-1 gD Protein

abx260325-5g 5 µg
EUR 350

Herpes Simplex Virus-2 gD Protein

abx260326-20g 20 µg
EUR 950

Herpes Simplex Virus-2 gD Protein

abx260326-5g 5 µg
EUR 350

Herpes Simplex Virus-2 gG Protein

abx260327-20g 20 µg
EUR 950

Herpes Simplex Virus-2 gG Protein

abx260327-5g 5 µg
EUR 350

Herpes Simplex Virus-1 gG Protein

abx260328-10g 10 µg
EUR 950

Herpes Simplex Virus-1 gG Protein

abx260328-2g 2 µg
EUR 350

Herpes Simplex Virus-2 gB Protein

abx260330-20g 20 µg
EUR 950

Herpes Simplex Virus-2 gB Protein

abx260330-5g 5 µg
EUR 350

Herpes simplex type 1 RT PCR kit

RTq-H426-100D 100T
EUR 860.4

Herpes simplex type 1 RT PCR kit

RTq-H426-150D 150T
EUR 969.6

Herpes simplex type 1 RT PCR kit

RTq-H426-50D 50T
EUR 717.6

Herpes simplex type 2 RT PCR kit

RTq-H427-100D 100T
EUR 860.4

Herpes simplex type 2 RT PCR kit

RTq-H427-150D 150T
EUR 969.6

Herpes simplex type 2 RT PCR kit

RTq-H427-50D 50T
EUR 717.6

Herpes Simplex Virus-2 VP22 Protein

20-abx260332
  • Ask for price
  • Ask for price
  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

Herpes Simplex Virus-2 VP22 Protein

abx260332-10g 10 µg
EUR 950

Herpes Simplex Virus-2 VP22 Protein

abx260332-2g 2 µg
EUR 350

Herpes simplex Virus 1 (HSV 1) IgG

DEHSV1G0500 96
EUR 92

Herpes simplex Virus 1 (HSV 1) IgM

DEHSV1M0500 96
EUR 92

Herpes simplex Virus 2 (HSV 2) IgG

DEHSV2G0540 96
EUR 92

Herpes simplex Virus 2 (HSV 2) IgM

DEHSV2M0540 96
EUR 92

Herpes simplex Virus 1+2 (HSV) IgG

DEHSVG0250 96
EUR 92

 

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